Miss Kepler is a science teacher at the school. She was played by Gene Raye Price.
- Barney in Outer Space (first appearance)
- Barney's Halloween Party
- Let's Go to the Zoo (last appearance)
- As she is a science teacher, her name refers to Johannes Kepler (founder of the three laws of planetary motion).
- In Once a Pond a Time, Curtis mentioned that his science teacher created a pond in the playground, but fails to mention a name.
- As revealed in Let's Go to the Zoo, her summer job is being a zookeeper.
- Barney wasn't seen speaking to Miss Kepler until Barney's Halloween Party.